This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0032174
PrimePCR™ PreAmp for SYBR® Green Assay: St6gal1, Mouse
PrimePCR™ Template for SYBR® Green Assay: St6gal1, Mouse
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein which is normally found in the Golgi but can be proteolytically processed to a soluble form is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6 CD75 and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene and a pseudogene of this gene is located on chromosome 15. [provided by RefSeq Nov 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.