PrimePCR™ SYBR® Green Assay: Gpr22, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0033129

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0009340
Assay Design:   Intron-spanning
Chromosome Location:   12:31709962-31713640question
Amplicon Length:   185
Splice Variants Targeted:   ENSMUST00000057783 ENSMUST00000176710 ENSMUST00000174480

Gene Information

Description Not Available

Gene Symbol:   Gpr22
Gene Name:   G protein-coupled receptor 22
Aliases:   2900068K05Rik, AW061316
RefSeq:   NC_000078.6 NT_039548.8
Ensembl:   ENSMUSG00000044067
Entrez:   73010
UniGene:   Mm.441517
Chromosome Mapping:   12 A3|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.310000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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