PrimePCR™ SYBR® Green Assay: Mark2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0033076

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0009212
Assay Design:   Intron-spanning
Chromosome Location:   19:7285885-7287002question
Amplicon Length:   183
Splice Variants Targeted:   ENSMUST00000032557 ENSMUST00000164205 ENSMUST00000025921 ENSMUST00000171721 ENSMUST00000165965 ENSMUST00000165286 ENSMUST00000168872 ENSMUST00000171393 ENSMUST00000051711

Gene Information

Description Not Available

Gene Symbol:   Mark2
Gene Name:   MAP/microtubule affinity-regulating kinase 2
Aliases:   AU024026, EMK-1, Emk, Par-1, Par-1b, mKIAA4207
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000024969
Entrez:   13728
UniGene:   Mm.258986
Chromosome Mapping:   19 B|19 3.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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