This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0029473
PrimePCR™ PreAmp for SYBR® Green Assay: Fxyd7, Mouse
PrimePCR™ Template for SYBR® Green Assay: Fxyd7, Mouse
This reference sequence was derived from multiple replicate ESTs and validated by similar human genomic sequence. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Transmembrane topology has been established for two family members (FXYD1 and FXYD2) with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. FXYD2 also known as the gamma subunit of the NaK-ATPase regulates the properties of that enzyme. FXYD1 (phospholemman) FXYD2 (gamma) FXYD3 (MAT-8) FXYD4 (CHIF) and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. This gene product FXYD7 is novel and has not been characterized as a protein. [RefSeq curation by Kathleen J. Sweadner Ph.D. email@example.com. Dec 2000]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.