PrimePCR™ SYBR® Green Assay: Sorbs2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0029980

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0006673
Assay Design:   Intron-spanning
Chromosome Location:   8:45789976-45792852question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000138049 ENSMUST00000135336 ENSMUST00000153798 ENSMUST00000139869 ENSMUST00000132139 ENSMUST00000130011 ENSMUST00000125295 ENSMUST00000140088 ENSMUST00000155858 ENSMUST00000146627 ENSMUST00000171337 ENSMUST00000067107 ENSMUST00000098788 ENSMUST00000067065

Gene Information

Description Not Available

Gene Symbol:   Sorbs2
Gene Name:   Sorbin and SH3 domain containing 2
Aliases:   2010203O03Rik, 9430041O17Rik, A530071H08, Argbp2, mKIAA0777
RefSeq:   NC_000074.6 NT_039460.8
Ensembl:   ENSMUSG00000031626
Entrez:   234214
UniGene:   Mm.211096
Chromosome Mapping:   8 B1.1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.090000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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