PrimePCR™ SYBR® Green Assay: Yap1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0030122

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0005990
Assay Design:   Intron-spanning
Chromosome Location:   9:7936584-7938509question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000086580 ENSMUST00000065353 ENSMUST00000174604

Gene Information

This gene encodes a protein which binds to the SH3 domain of the Yes proto-oncogene product a tyrosine kinase. This protein contains a WW domain consisting of four conserved aromatic amino acids including two tryptophan residues. This conserved WW domain is found in various structural regulatory and signaling molecules in various species and may play a role in protein-protein interaction. Following cellular damage phosphorylation of this encoded protein may suppress apoptosis. This protein may be involved in malignant transformation in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   Yap1
Gene Name:   Yes-associated protein 1
Aliases:   AI325207, Yap, Yap65, Yki, Yorkie
RefSeq:   NC_000075.6 NT_039471.8
Ensembl:   ENSMUSG00000053110
Entrez:   22601
UniGene:   Mm.221992
Chromosome Mapping:   9 A1|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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