PrimePCR™ Probe Assay: Apol7b, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062886
Assay Design:   exonic
Chromosome Location:   15:77423191-77423307question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000171796 ENSMUST00000171556 ENSMUST00000112958 ENSMUST00000086819 ENSMUST00000118835 ENSMUST00000093534 ENSMUST00000051379 ENSMUST00000059115 ENSMUST00000176074 ENSMUST00000175919 ENSMUST00000010745 ENSMUST00000089469 ENSMUST00000062562 ENSMUST00000096358

Gene Information

Description Not Available

Gene Symbol:   Apol7b
Gene Name:   Apolipoprotein L 7b
Aliases:   Not Available
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000068252
Entrez:   278679
UniGene:   Mm.440965
Chromosome Mapping:   15 E1|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.150000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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