PrimePCR™ Probe Assay: Vmn2r89, Mouse

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062862
Assay Design:   exonic
Chromosome Location:   14:51460452-51460560question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000163019 ENSMUST00000159674 ENSMUST00000022438 ENSMUST00000159611 ENSMUST00000159734 ENSMUST00000164918 ENSMUST00000094502 ENSMUST00000171811 ENSMUST00000170257 ENSMUST00000164227 ENSMUST00000096791 ENSMUST00000071277 ENSMUST00000179115 ENSMUST00000178970 ENSMUST00000099422 ENSMUST00000079163 ENSMUST00000053253 ENSMUST00000170341 ENSMUST00000167133 ENSMUST00000165180 ENSMUST00000171841 ENSMUST00000096792 ENSMUST00000074107

Gene Information

Description Not Available

Gene Symbol:   Vmn2r89
Gene Name:   Vomeronasal 2, receptor 89
Aliases:   V2r10, V2r11
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000070448
Entrez:   22301
UniGene:   Mm.482572
Chromosome Mapping:   14 C1|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.970000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.