PrimePCR™ Probe Assay: CR974575.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CR974575.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CR974575.1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062845
Assay Design:   exonic
Chromosome Location:   12:34290508-34290631question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000161938 ENSMUST00000076842 ENSMUST00000050556 ENSMUST00000170335 ENSMUST00000152407 ENSMUST00000054289 ENSMUST00000146867 ENSMUST00000170715 ENSMUST00000032811 ENSMUST00000094617 ENSMUST00000071458 ENSMUST00000095471 ENSMUST00000165165 ENSMUST00000166546 ENSMUST00000088373 ENSMUST00000113169 ENSMUST00000179802 ENSMUST00000090257 ENSMUST00000112642 ENSMUST00000000449 ENSMUST00000169678 ENSMUST00000085368 ENSMUST00000177599 ENSMUST00000118755 ENSMUST00000094026 ENSMUST00000042647

Gene Information

Description Not Available

Gene Symbol:   CR974575.1
Gene Name:   Not available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095406
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 35.640000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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