PrimePCR™ Probe Assay: Vmn1r14, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062747
Assay Design:   exonic
Chromosome Location:   6:57234110-57234222question
Amplicon Length:   83
Splice Variants Targeted:   ENSMUST00000176838 ENSMUST00000096612 ENSMUST00000176954 ENSMUST00000078885 ENSMUST00000176376 ENSMUST00000071304 ENSMUST00000177267 ENSMUST00000081186 ENSMUST00000177435 ENSMUST00000175817 ENSMUST00000168416 ENSMUST00000078890 ENSMUST00000079584 ENSMUST00000074381 ENSMUST00000071414

Gene Information

Description Not Available

Gene Symbol:   Vmn1r14
Gene Name:   Vomeronasal 1 receptor 14
Aliases:   V1rc7
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000093692
Entrez:   113864
UniGene:   Mm.431974
Chromosome Mapping:   6 B3|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 35.310000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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