PrimePCR™ Probe Assay: AC239604.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC239604.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC239604.1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062708
Assay Design:   exonic
Chromosome Location:   8:21427467-21427571question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000168645 ENSMUST00000064718 ENSMUST00000076951 ENSMUST00000110758 ENSMUST00000076754 ENSMUST00000098899 ENSMUST00000078121 ENSMUST00000077452 ENSMUST00000098898 ENSMUST00000098897 ENSMUST00000098896 ENSMUST00000110754 ENSMUST00000098895 ENSMUST00000098893 ENSMUST00000098892 ENSMUST00000098890 ENSMUST00000170275 ENSMUST00000120874 ENSMUST00000095424 ENSMUST00000110752 ENSMUST00000084042 ENSMUST00000084041 ENSMUST00000084040 ENSMUST00000110749 ENSMUST00000074343 ENSMUST00000079528 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC239604.1
Gene Name:   Predicted gene 14850 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000094662
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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