PrimePCR™ Probe Assay: Rell2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rell2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rell2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062704
Assay Design:   exonic
Chromosome Location:   18:37958024-37958170question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000047786 ENSMUST00000099752 ENSMUST00000118171 ENSMUST00000034225 ENSMUST00000148078 ENSMUST00000141250 ENSMUST00000035548 ENSMUST00000176902 ENSMUST00000176104 ENSMUST00000070709 ENSMUST00000177058 ENSMUST00000163591 ENSMUST00000091932 ENSMUST00000169498 ENSMUST00000043437 ENSMUST00000037763 ENSMUST00000125192 ENSMUST00000100375 ENSMUST00000058793 ENSMUST00000042601 ENSMUST00000060389 ENSMUST00000121101 ENSMUST00000098483 ENSMUST00000067258 ENSMUST00000167574 ENSMUST00000038389 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rell2
Gene Name:   RELT-like 2
Aliases:   4631403P03Rik, ependolin
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000044024
Entrez:   225392
Chromosome Mapping:   18 B3|18

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.060000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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