PrimePCR™ Probe Assay: Defa22, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062701
Assay Design:   exonic
Chromosome Location:   8:21162306-21162446question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000110758 ENSMUST00000076754 ENSMUST00000098899 ENSMUST00000078121 ENSMUST00000098898 ENSMUST00000098897 ENSMUST00000110754 ENSMUST00000098893 ENSMUST00000098890 ENSMUST00000120874 ENSMUST00000110752 ENSMUST00000084042 ENSMUST00000084041 ENSMUST00000084040 ENSMUST00000110749 ENSMUST00000079528 ENSMUST00000071886 ENSMUST00000080533 ENSMUST00000020617

Gene Information

Description Not Available

Gene Symbol:   Defa22
Gene Name:   Defensin, alpha, 22
Aliases:   Defcr22
RefSeq:   NC_000074.6 NT_039457.8
Ensembl:   ENSMUSG00000074443
Entrez:   382059
UniGene:   Mm.42715
Chromosome Mapping:   8 A2|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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