PrimePCR™ Probe Assay: Obox5, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062672
Assay Design:   exonic
Chromosome Location:   7:15757943-15758543question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000172478 ENSMUST00000036575 ENSMUST00000174076 ENSMUST00000174305 ENSMUST00000172881 ENSMUST00000067288 ENSMUST00000172463 ENSMUST00000173443 ENSMUST00000173245 ENSMUST00000181001 ENSMUST00000174443 ENSMUST00000173912 ENSMUST00000173395 ENSMUST00000174151 ENSMUST00000174842 ENSMUST00000086122 ENSMUST00000095217 ENSMUST00000173455 ENSMUST00000098802 ENSMUST00000173053 ENSMUST00000108513 ENSMUST00000109627 ENSMUST00000109625 ENSMUST00000143764 ENSMUST00000122383 ENSMUST00000106582 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Obox5
Gene Name:   Oocyte specific homeobox 5
Aliases:   C87544
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000074366
Entrez:   252829
UniGene:   Mm.222738
Chromosome Mapping:   7 A2|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.550000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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