PrimePCR™ Probe Assay: Olfr969, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best efficiency

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Olfr969, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olfr969, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0062616
Assay Design:   exonic
Chromosome Location:   9:39795470-39795614question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000086062 ENSMUST00000104874 ENSMUST00000058789 ENSMUST00000075680 ENSMUST00000055567 ENSMUST00000057811 ENSMUST00000071617 ENSMUST00000079650 ENSMUST00000051653 ENSMUST00000075928 ENSMUST00000074566 ENSMUST00000075467 ENSMUST00000079767 ENSMUST00000076802 ENSMUST00000099904 ENSMUST00000099892 ENSMUST00000105213

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   Olfr969
Gene Name:   Olfactory receptor 969
Aliases:   MOR171-7
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000094254
Entrez:   258823
Chromosome Mapping:   9 A5-B|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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