PrimePCR™ Probe Assay: Myl6, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Myl6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Myl6, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060677
Assay Design:   exonic
Chromosome Location:   10:128490922-128492062question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000132517 ENSMUST00000171637 ENSMUST00000043866 ENSMUST00000053175 ENSMUST00000100721 ENSMUST00000170242 ENSMUST00000168839 ENSMUST00000071935 ENSMUST00000001583 ENSMUST00000091851 ENSMUST00000159162 ENSMUST00000026494 ENSMUST00000117100 ENSMUST00000022851 ENSMUST00000022036 ENSMUST00000055539 ENSMUST00000105244 ENSMUST00000105242 ENSMUST00000105245 ENSMUST00000164181 ENSMUST00000056915 ENSMUST00000042850 ENSMUST00000002452 ENSMUST00000106804 ENSMUST00000106803 ENSMUST00000068693 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Myl6
Gene Name:   Myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
Aliases:   ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3SM, MLC3nm, Myln
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000090841
Entrez:   17904
Chromosome Mapping:   10 D3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.650000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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