PrimePCR™ Probe Assay: Klra10, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Klra10, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Klra10, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060625
Assay Design:   exonic
Chromosome Location:   6:130281827-130281927question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000110081 ENSMUST00000110078 ENSMUST00000122219 ENSMUST00000014687 ENSMUST00000118060 ENSMUST00000014683 ENSMUST00000074056 ENSMUST00000112032 ENSMUST00000071554 ENSMUST00000049304 ENSMUST00000088011 ENSMUST00000032286 ENSMUST00000112013 ENSMUST00000112020 ENSMUST00000111998 ENSMUST00000088017 ENSMUST00000032288 ENSMUST00000032306 ENSMUST00000088867 ENSMUST00000061201 ENSMUST00000043983 ENSMUST00000043325

Gene Information

Description Not Available

Gene Symbol:   Klra10
Gene Name:   Killer cell lectin-like receptor subfamily A, member 10
Aliases:   Ly49J, Ly49i2
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000072718
Entrez:   16628
Chromosome Mapping:   6 F3|6 62.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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