PrimePCR™ Probe Assay: Ssbp4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Ssbp4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ssbp4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060611
Assay Design:   exonic
Chromosome Location:   8:70597540-70597762question
Amplicon Length:   193
Splice Variants Targeted:   ENSMUST00000074991 ENSMUST00000178522 ENSMUST00000095737 ENSMUST00000033532 ENSMUST00000088311 ENSMUST00000116375 ENSMUST00000066658 ENSMUST00000168593 ENSMUST00000000641 ENSMUST00000113982 ENSMUST00000049908

Gene Information

Description Not Available

Gene Symbol:   Ssbp4
Gene Name:   Single stranded DNA binding protein 4
Aliases:   1210002E11Rik, AW743380, Sspb4
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000070003
Entrez:   76900
Chromosome Mapping:   8|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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