PrimePCR™ Probe Assay: Nnat, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060598
Assay Design:   exonic
Chromosome Location:   2:157560165-157561219question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000153739 ENSMUST00000173595 ENSMUST00000109526 ENSMUST00000173839 ENSMUST00000173041 ENSMUST00000173793 ENSMUST00000172487 ENSMUST00000088484 ENSMUST00000087923

Gene Information

Description Not Available

Gene Symbol:   Nnat
Gene Name:   Neuronatin
Aliases:   5730414I02Rik, AW107673, Peg5
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000067786
Entrez:   18111
UniGene:   Mm.34330
Chromosome Mapping:   2 H1|2 88.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.110000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download