PrimePCR™ Probe Assay: Peg10, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Peg10, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Peg10, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059721
Assay Design:   exonic
Chromosome Location:   6:4755445-4755560question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000176204 ENSMUST00000176551 ENSMUST00000166678

Gene Information

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs) RF1 and RF1/RF2 as well as retroviral-like slippage and pseudoknot elements which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Knockout studies in mice suggest that this gene is critical for parthenogenetic development. [provided by RefSeq Feb 2010]

Gene Symbol:   Peg10
Gene Name:   Paternally expressed 10
Aliases:   AA407948, Edr, HB-1, MEF3L, Mar2, Mart2, MyEF-3
RefSeq:   NC_000072.6 NT_039340.7
Ensembl:   ENSMUSG00000092035
Entrez:   170676
Chromosome Mapping:   6 A1|6 0.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.040000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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