PrimePCR™ Probe Assay: Vmn1r181, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059336
Assay Design:   exonic
Chromosome Location:   7:23984059-23984267question
Amplicon Length:   179
Splice Variants Targeted:   ENSMUST00000098736 ENSMUST00000177774 ENSMUST00000049819 ENSMUST00000077386

Gene Information

Description Not Available

Gene Symbol:   Vmn1r181
Gene Name:   Vomeronasal 1 receptor 181
Aliases:   V1rd20
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000057946
Entrez:   404289
UniGene:   Mm.279185
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.870000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download