PrimePCR™ Probe Assay: Wdfy4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059314
Assay Design:   exonic
Chromosome Location:   14:33162845-33162949question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000130509 ENSMUST00000159606 ENSMUST00000061753 ENSMUST00000096257 ENSMUST00000046603 ENSMUST00000001480 ENSMUST00000109261 ENSMUST00000109260

Gene Information

Description Not Available

Gene Symbol:   Wdfy4
Gene Name:   WD repeat and FYVE domain containing 4
Aliases:   Not Available
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000051506
Entrez:   545030
UniGene:   Mm.318345
Chromosome Mapping:   14 B|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download