PrimePCR™ Probe Assay: Defb6, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Defb6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Defb6, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059313
Assay Design:   exonic
Chromosome Location:   8:19225483-19227992question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000148171 ENSMUST00000163321 ENSMUST00000041924 ENSMUST00000053568 ENSMUST00000114256 ENSMUST00000114247 ENSMUST00000081017 ENSMUST00000063112 ENSMUST00000021428 ENSMUST00000034285 ENSMUST00000025830 ENSMUST00000049621 ENSMUST00000107273 ENSMUST00000088811 ENSMUST00000037260 ENSMUST00000037117 ENSMUST00000171404 ENSMUST00000069711

Gene Information

Description Not Available

Gene Symbol:   Defb6
Gene Name:   Defensin beta 6
Aliases:   BD-6
RefSeq:   NC_000074.6 NT_039455.8
Ensembl:   ENSMUSG00000050756
Entrez:   116746
Chromosome Mapping:   8 A1.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.050000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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