PrimePCR™ Probe Assay: Scml4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059303
Assay Design:   exonic
Chromosome Location:   10:42912202-42924162question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000106162 ENSMUST00000030673 ENSMUST00000030170 ENSMUST00000163852 ENSMUST00000126319 ENSMUST00000077816 ENSMUST00000022629 ENSMUST00000023779 ENSMUST00000063063 ENSMUST00000157071 ENSMUST00000105495 ENSMUST00000105494 ENSMUST00000136841 ENSMUST00000125576 ENSMUST00000030187 ENSMUST00000087978 ENSMUST00000035371

Gene Information

Description Not Available

Gene Symbol:   Scml4
Gene Name:   Sex comb on midleg-like 4 (Drosophila)
Aliases:   9330161D17, mFLJ00197
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000044770
Entrez:   268297
UniGene:   Mm.98731
Chromosome Mapping:   10 B2|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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