PrimePCR™ Probe Assay: Pla2g4e, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059037
Assay Design:   exonic
Chromosome Location:   2:120167152-120167293question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000090071

Gene Information

Description Not Available

Gene Symbol:   Pla2g4e
Gene Name:   Phospholipase A2, group IVE
Aliases:   2310026J01Rik, C230096D22, Pla2epsilon
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000050211
Entrez:   329502
UniGene:   Mm.330505
Chromosome Mapping:   2 E5|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.200000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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