PrimePCR™ Probe Assay: Musk, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0058316
Assay Design:   exonic
Chromosome Location:   4:58344158-58344283question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000102893 ENSMUST00000084578 ENSMUST00000081919 ENSMUST00000098057 ENSMUST00000179951 ENSMUST00000098059 ENSMUST00000177951

Gene Information

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development including organization of the postsynaptic membrane synaptic gene transcription patterning of skeletal muscle anchoring of acetylcholinesterase and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   Musk
Gene Name:   Muscle, skeletal, receptor tyrosine kinase
Aliases:   Mdk4, Mlk, Nsk1, Nsk2, Nsk3
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000057280
Entrez:   18198
UniGene:   Mm.16148
Chromosome Mapping:   4 B3|4 26.3 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download