PrimePCR™ Probe Assay: Klhdc4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: Klhdc4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Klhdc4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057958
Assay Design:   exonic
Chromosome Location:   8:121821357-121829390question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000003469 ENSMUST00000003370 ENSMUST00000109799 ENSMUST00000004156 ENSMUST00000045884 ENSMUST00000174717 ENSMUST00000029510 ENSMUST00000103119 ENSMUST00000105113 ENSMUST00000108901 ENSMUST00000087563 ENSMUST00000051772 ENSMUST00000074834 ENSMUST00000023226 ENSMUST00000072692 ENSMUST00000169438 ENSMUST00000169714 ENSMUST00000169108 ENSMUST00000076442 ENSMUST00000171562 ENSMUST00000165210 ENSMUST00000089610 ENSMUST00000071869 ENSMUST00000073418 ENSMUST00000054449 ENSMUST00000080857 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Klhdc4
Gene Name:   Kelch domain containing 4
Aliases:   AA408426, AV352552, BC012312, G430025P05Rik
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000040263
Entrez:   234825
Chromosome Mapping:   8 E1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.210000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download