PrimePCR™ Probe Assay: Zranb3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057821
Assay Design:   exonic
Chromosome Location:   1:128040873-128040974question
Amplicon Length:   72
Splice Variants Targeted:   ENSMUST00000086614 ENSMUST00000097597 ENSMUST00000112538

Gene Information

Description Not Available

Gene Symbol:   Zranb3
Gene Name:   Zinc finger, RAN-binding domain containing 3
Aliases:   4933425L19Rik, AI316834, C730006D09
RefSeq:   NC_000067.6 NT_078297.7
Ensembl:   ENSMUSG00000036086
Entrez:   226409
UniGene:   Mm.425048
Chromosome Mapping:   1 E4|1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.400000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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