PrimePCR™ Probe Assay: Zfp57, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057814
Assay Design:   exonic
Chromosome Location:   17:37004798-37004895question
Amplicon Length:   68
Splice Variants Targeted:   ENSMUST00000089968 ENSMUST00000172527 ENSMUST00000069250 ENSMUST00000174747 ENSMUST00000173921 ENSMUST00000172580 ENSMUST00000174524 ENSMUST00000174672 ENSMUST00000172540

Gene Information

Description Not Available

Gene Symbol:   Zfp57
Gene Name:   Zinc finger protein 57
Aliases:   G19, Zfp-57
RefSeq:   NC_000083.6 NT_039649.8 NT_187004.1 NT_187009.1 NT_187027.1
Ensembl:   ENSMUSG00000036036
Entrez:   22715
UniGene:   Mm.305561
Chromosome Mapping:   17 B3|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.810000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download