PrimePCR™ Probe Assay: Tmem106a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057757
Assay Design:   exonic
Chromosome Location:   11:101583665-101583784question
Amplicon Length:   90
Splice Variants Targeted:   ENSMUST00000039581 ENSMUST00000100403 ENSMUST00000107194 ENSMUST00000128614

Gene Information

Description Not Available

Gene Symbol:   Tmem106a
Gene Name:   Transmembrane protein 106A
Aliases:   0610008L10Rik, AI043106, BC022145
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000034947
Entrez:   217203
UniGene:   Mm.280559
Chromosome Mapping:   11 D|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download