PrimePCR™ Probe Assay: Ppp2r4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057663
Assay Design:   exonic
Chromosome Location:   2:30446521-30446615question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000042055 ENSMUST00000113601 ENSMUST00000113603 ENSMUST00000131476 ENSMUST00000136009

Gene Information

Description Not Available

Gene Symbol:   Ppp2r4
Gene Name:   Protein phosphatase 2A, regulatory subunit B (PR 53)
Aliases:   2610042B21Rik, C77440, N28142, PR53
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000039515
Entrez:   110854
UniGene:   Mm.275393
Chromosome Mapping:   2 B

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.590000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download