PrimePCR™ Probe Assay: Lphn3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Lphn3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Lphn3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056967
Assay Design:   exonic
Chromosome Location:   5:81688557-81688678question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000117253 ENSMUST00000120128 ENSMUST00000121707 ENSMUST00000118078 ENSMUST00000120144 ENSMUST00000120445 ENSMUST00000120673 ENSMUST00000118034 ENSMUST00000118442 ENSMUST00000121641 ENSMUST00000117985 ENSMUST00000120292 ENSMUST00000117407 ENSMUST00000119385 ENSMUST00000122356 ENSMUST00000122037 ENSMUST00000119788 ENSMUST00000124117 ENSMUST00000036068 ENSMUST00000072521

Gene Information

Description Not Available

Gene Symbol:   Lphn3
Gene Name:   Latrophilin 3
Aliases:   5430402I23Rik, CIRL-3, D130075K09Rik, Gm1379, LEC3, mKIAA0768
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000037605
Entrez:   319387
Chromosome Mapping:   5 D-E1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download