PrimePCR™ Probe Assay: Etl4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056835
Assay Design:   exonic
Chromosome Location:   2:20743856-20743987question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000114610 ENSMUST00000045555 ENSMUST00000114614 ENSMUST00000114604 ENSMUST00000066509 ENSMUST00000114607 ENSMUST00000114606 ENSMUST00000114608 ENSMUST00000114627

Gene Information

Description Not Available

Gene Symbol:   Etl4
Gene Name:   Enhancer trap locus 4
Aliases:   6620402G01Rik, 9430077C05Rik, BC026657, E330027G05Rik, Etl-4, Skt
RefSeq:   NC_000068.7 NT_039202.8
Ensembl:   ENSMUSG00000036617
Entrez:   208618
UniGene:   Mm.237935
Chromosome Mapping:   2 A3|2 9.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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