PrimePCR™ Probe Assay: Abcg4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Abcg4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Abcg4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056578
Assay Design:   exonic
Chromosome Location:   9:44287245-44288014question
Amplicon Length:   192
Splice Variants Targeted:   ENSMUST00000066285 ENSMUST00000034648 ENSMUST00000161354 ENSMUST00000161408 ENSMUST00000160384 ENSMUST00000101377 ENSMUST00000101382 ENSMUST00000101376 ENSMUST00000069809 ENSMUST00000080673 ENSMUST00000091818 ENSMUST00000088246 ENSMUST00000084954 ENSMUST00000108568 ENSMUST00000079056 ENSMUST00000102564 ENSMUST00000124943 ENSMUST00000150076 ENSMUST00000102563 ENSMUST00000080036

Gene Information

Description Not Available

Gene Symbol:   Abcg4
Gene Name:   ATP-binding cassette, sub-family G (WHITE), member 4
Aliases:   6430517O04Rik
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000032131
Entrez:   192663
Chromosome Mapping:   9|9 A5.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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