PrimePCR™ Probe Assay: Rchy1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rchy1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rchy1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055758
Assay Design:   exonic
Chromosome Location:   5:91949643-91949781question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000031345 ENSMUST00000169948

Gene Information

This gene encodes a protein containing CHY- CTCHY- and RING-type zinc-fingers. The encoded protein functions as an E3 ubiquitin ligase and mediates the degradation of target proteins such as p53. The activity of this protein is important in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2012]

Gene Symbol:   Rchy1
Gene Name:   Ring finger and CHY zinc finger domain containing 1
Aliases:   6720407C15Rik, ARNIP, AU042618, CHIMP, PRO1996, Pirh2, Zfp363, mARNIP
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000029397
Entrez:   68098
Chromosome Mapping:   5 E2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.600000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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