PrimePCR™ Probe Assay: Gne, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Gne, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gne, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055515
Assay Design:   exonic
Chromosome Location:   4:44060098-44066760question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000030201 ENSMUST00000102936 ENSMUST00000173234 ENSMUST00000173274 ENSMUST00000144985 ENSMUST00000173383 ENSMUST00000128439 ENSMUST00000140724

Gene Information

Description Not Available

Gene Symbol:   Gne
Gene Name:   Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases:   2310066H07Rik, DMRV, GLCNE, IBM2, NM, Uae1
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000028479
Entrez:   50798
Chromosome Mapping:   4 B1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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