PrimePCR™ Probe Assay: Adam15, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055274
Assay Design:   exonic
Chromosome Location:   3:89339811-89339945question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000107448 ENSMUST00000029676 ENSMUST00000107446 ENSMUST00000074582

Gene Information

Description Not Available

Gene Symbol:   Adam15
Gene Name:   A disintegrin and metallopeptidase domain 15 (metargidin)
Aliases:   MDC15, metargidin
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000028041
Entrez:   11490
UniGene:   Mm.274049
Chromosome Mapping:   3 F1|3 44.4 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.060000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download