PrimePCR™ Probe Assay: Rapgef3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055244
Assay Design:   exonic
Chromosome Location:   15:97745266-97745400question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000126854 ENSMUST00000129223 ENSMUST00000128775 ENSMUST00000177352 ENSMUST00000134885

Gene Information

Description Not Available

Gene Symbol:   Rapgef3
Gene Name:   Rap guanine nucleotide exchange factor (GEF) 3
Aliases:   2310016P22Rik, 9330170P05Rik, Epac, Epac1
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022469
Entrez:   223864
UniGene:   Mm.24028
Chromosome Mapping:   15 F1|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download