PrimePCR™ Probe Assay: Pigq, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055228
Assay Design:   exonic
Chromosome Location:   17:25937280-25937398question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000097368 ENSMUST00000026823 ENSMUST00000139226 ENSMUST00000145745 ENSMUST00000148307 ENSMUST00000148382 ENSMUST00000032143 ENSMUST00000105256 ENSMUST00000026209

Gene Information

Description Not Available

Gene Symbol:   Pigq
Gene Name:   Phosphatidylinositol glycan anchor biosynthesis, class Q
Aliases:   Gpi1, Gpi1h, Gpi1p, Gpih
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000025728
Entrez:   14755
UniGene:   Mm.362054
Chromosome Mapping:   17|17 B

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download