PrimePCR™ Probe Assay: Asap1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Asap1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Asap1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054655
Assay Design:   exonic
Chromosome Location:   15:64191494-64191625question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000177371 ENSMUST00000177083 ENSMUST00000177374 ENSMUST00000175793 ENSMUST00000175799 ENSMUST00000176384 ENSMUST00000177035 ENSMUST00000176014 ENSMUST00000176358 ENSMUST00000023008 ENSMUST00000110115 ENSMUST00000110114

Gene Information

Description Not Available

Gene Symbol:   Asap1
Gene Name:   ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Aliases:   AV239055, DEF-1, Ddef1, PAP, mKIAA1249., s19
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000022377
Entrez:   13196
Chromosome Mapping:   15|15 D3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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