PrimePCR™ Probe Assay: Slc30a6, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054477
Assay Design:   exonic
Chromosome Location:   17:74423534-74423645question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000179074 ENSMUST00000024870

Gene Information

Description Not Available

Gene Symbol:   Slc30a6
Gene Name:   Solute carrier family 30 (zinc transporter), member 6
Aliases:   9530029F08Rik, ZnT6
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000024069
Entrez:   210148
UniGene:   Mm.243943
Chromosome Mapping:   17 E2|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.070000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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