PrimePCR™ Probe Assay: Mrvi1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053732
Assay Design:   exonic
Chromosome Location:   7:110923949-110924070question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000127935 ENSMUST00000005751 ENSMUST00000125758 ENSMUST00000142368

Gene Information

This gene is a putative tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein which is found in the membrane of the endoplasmic reticulum is similar to Jaw1 a lymphoid-restricted protein whose expression is downregulated during myeloid differentiation. Studies in mouse suggest that MRV integration at this locus induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Additional isoforms may arise through the use of alternative non-AUG (CUG) translation initiation site. [provided by RefSeq Jul 2008]

Gene Symbol:   Mrvi1
Gene Name:   MRV integration site 1
Aliases:   BB115629, Irag, Ris1
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000005611
Entrez:   17540
UniGene:   Mm.250023
Chromosome Mapping:   7 F1|7 51.52 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download