PrimePCR™ Probe Assay: D17Wsu104e, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053714
Assay Design:   exonic
Chromosome Location:   17:56176701-56176836question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000019723

Gene Information

The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was identified in error as interleukin 25. This activity has not been reproducible however and the function of this protein is currently unknown. [provided by RefSeq Jul 2008]

Gene Symbol:   D17Wsu104e
Gene Name:   DNA segment, Chr 17, Wayne State University 104, expressed
Aliases:   IL-25, Il25, Ly6elg
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000019579
Entrez:   28106
UniGene:   Mm.294870
Chromosome Mapping:   17 D|17 37.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.620000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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