PrimePCR™ Probe Assay: Klc1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Klc1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Klc1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053412
Assay Design:   exonic
Chromosome Location:   12:111789350-111789463question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000084941 ENSMUST00000118471 ENSMUST00000122300 ENSMUST00000120544 ENSMUST00000134578

Gene Information

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles mitochondria and the Golgi complex. Thus kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2" this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   Klc1
Gene Name:   Kinesin light chain 1
Aliases:   AI874768, Kns2
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000021288
Entrez:   16593
Chromosome Mapping:   12 F1|12 57.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.790000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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