PrimePCR™ Probe Assay: Suz12, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Suz12, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Suz12, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053136
Assay Design:   exonic
Chromosome Location:   11:80029181-80029304question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000017692 ENSMUST00000163272

Gene Information

This gene encodes a core component of the polycomb repressive complex 2 (PRC2) that also includes at least embryonic ectoderm development protein (EED) and enhancer of zeste homolog 1 or 2 (EZH1 or EZH2). Through the methyltransferase activity of EZH1 or EZH2 the PRC2 complex methylates Lys9 and Lys27 of histone 3 and Lys26 of histone 1 leading to recruitment of the PRC1 complex histone 2A ubiquitylation and transcriptional repression of the target genes. This gene product is essential for the activity and integrity of the PRC2 complex and is required for X chromosome inactivation stem cell maintenance and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   Suz12
Gene Name:   Suppressor of zeste 12 homolog (Drosophila)
Aliases:   2610028O16Rik, AI195385, AU016842, AW536442, D11Ertd530e, mKIAA0160
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017548
Entrez:   52615
Chromosome Mapping:   11 B5|11 47.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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