This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Snrnp200, Mouse
PrimePCR™ Template for Probe Assay: Snrnp200, Mouse
On February 19 2002 this locus was switched from human to mouse. The source accession Z70200.1 is almost identical to the mouse BAC clone AC074224 and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.