PrimePCR™ Probe Assay: AC190166.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC190166.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC190166.1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0044077
Assay Design:   exonic
Chromosome Location:   Y:13223660-13223764question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000179125 ENSMUST00000177579 ENSMUST00000178559 ENSMUST00000177612 ENSMUST00000177878 ENSMUST00000178669 ENSMUST00000179612 ENSMUST00000179284 ENSMUST00000178050 ENSMUST00000178519 ENSMUST00000178428 ENSMUST00000178689 ENSMUST00000179522 ENSMUST00000179900 ENSMUST00000179219 ENSMUST00000179302 ENSMUST00000039064 ENSMUST00000171795 ENSMUST00000072406 ENSMUST00000164342 ENSMUST00000012440 ENSMUST00000180283 ENSMUST00000107498 ENSMUST00000090942 ENSMUST00000081695 ENSMUST00000109252 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC190166.1
Gene Name:   Serine-rich secreted Y-linked protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095759
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.430000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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