PrimePCR™ Probe Assay: Nudt1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0044026
Assay Design:   exonic
Chromosome Location:   5:140337645-140337829question
Amplicon Length:   155
Splice Variants Targeted:   ENSMUST00000003574 ENSMUST00000094821 ENSMUST00000128570 ENSMUST00000162849 ENSMUST00000037232 ENSMUST00000178977 ENSMUST00000179939 ENSMUST00000177974 ENSMUST00000031423 ENSMUST00000110299 ENSMUST00000112185 ENSMUST00000112183 ENSMUST00000043283 ENSMUST00000086564 ENSMUST00000155908 ENSMUST00000178440 ENSMUST00000051169 ENSMUST00000128901 ENSMUST00000036376 ENSMUST00000100387 ENSMUST00000071881 ENSMUST00000050205 ENSMUST00000110827 ENSMUST00000110826

Gene Information

Description Not Available

Gene Symbol:   Nudt1
Gene Name:   Nudix (nucleoside diphosphate linked moiety X)-type motif 1
Aliases:   Mth1
RefSeq:   NC_000071.6 NT_039316.8
Ensembl:   ENSMUSG00000036639
Entrez:   17766
UniGene:   Mm.118846
Chromosome Mapping:   5|5 G1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.680000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download