PrimePCR™ Probe Assay: Vmn2r98, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043993
Assay Design:   exonic
Chromosome Location:   17:19080662-19080775question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000094502 ENSMUST00000171811 ENSMUST00000170257 ENSMUST00000164227 ENSMUST00000165781 ENSMUST00000176802 ENSMUST00000172359 ENSMUST00000169686 ENSMUST00000172190 ENSMUST00000079206 ENSMUST00000166327 ENSMUST00000177244 ENSMUST00000165692 ENSMUST00000168710 ENSMUST00000170424 ENSMUST00000176107 ENSMUST00000166081 ENSMUST00000171914 ENSMUST00000171741 ENSMUST00000172203 ENSMUST00000168050 ENSMUST00000167382 ENSMUST00000167464 ENSMUST00000042090 ENSMUST00000167093 ENSMUST00000165180 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn2r98
Gene Name:   Vomeronasal 2, receptor 98
Aliases:   EG224552
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000096717
Entrez:   224552
UniGene:   Mm.469860
Chromosome Mapping:   17 A3.2|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 38.300000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download