PrimePCR™ Probe Assay: AC192334.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC192334.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC192334.1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043937
Assay Design:   exonic
Chromosome Location:   13:66119641-66119974question
Amplicon Length:   103
Splice Variants Targeted:   ENSMUST00000108007 ENSMUST00000122450 ENSMUST00000113729 ENSMUST00000177714 ENSMUST00000071300 ENSMUST00000074369 ENSMUST00000105315 ENSMUST00000085664 ENSMUST00000099439 ENSMUST00000105314 ENSMUST00000041264 ENSMUST00000107989 ENSMUST00000076195 ENSMUST00000101547 ENSMUST00000091563 ENSMUST00000167516 ENSMUST00000092528 ENSMUST00000173583 ENSMUST00000172579 ENSMUST00000091541 ENSMUST00000071320 ENSMUST00000079229 ENSMUST00000108015 ENSMUST00000071804 ENSMUST00000098509 ENSMUST00000073410 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC192334.1
Gene Name:   Zinc finger protein family member
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095909
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.960000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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